Journal: eNeuro
Article Title: Cortical Spreading Depression Promotes Persistent Mechanical Sensitization of Intracranial Meningeal Afferents: Implications for the Intracranial Mechanosensitivity of Migraine
doi: 10.1523/ENEURO.0287-16.2016
Figure Lengend Snippet: Experimental setup. Three skull openings (red ovals) were made. A small burr hole was made over the left frontal cortex to elicit CSD events using a pinprick (PP). Meningeal afferent activity was recorded in the left trigeminal ganglion (TG) using a tungsten microelectrode inserted through a craniotomy made over the contralateral hemisphere. An ipsilateral craniotomy was made to expose a part of the left transverse sinus (TS) and its vicinity to search for meningeal afferents with mechanical RF. Quantitative mechanical stimuli were delivered to the afferents’ RF using a feedback-controlled mechanical stimulator. Laser Doppler flowmetry (LDF) probe was placed over the cortex near the stimulated afferent’s RF to validate the induction of the CSD by testing related changes in cerebral blood flow. SSS, superior sagittal sinus.
Article Snippet: Mechanical responsiveness was quantitatively determined in each afferent by recording the responses to mechanical stimuli (100-ms rise time, 2-s width, 120-s interstimulus interval) delivered using a feedback-controlled mechanical stimulator (Series 300B, Aurora Scientific, Aurora, ON, Canada) and a custom-written script for Spike 2.
Techniques: Activity Assay